Introduction: somatic cell technology plays vital action for the generation of organism and polyclonal protein.
Here, antibody production are mentioned solely. during this method, a standard B-cell and a neoplastic cell are used. B-cell has protein production capability and malignant tumor cell has immortality and high proliferation rate. created antibodies are specific in action. So, Identical antibodies production method is understood as somatic cell technology. The method includes six stages.1. protection :First, mice is vaccinated.
Then protein is created against the protection within the mice’s body. once the number of protein is optimum within the mice. it’s sacrificed and spleenocytes are taken out from it. Spleenocyte contains protein manufacturing B-cell. 2. Fusion :Here, the spleenocyte is united with malignant tumor cell.
50% synthetic resin glycol(PEG) is employed to fuse the cell. united cell is referred as somatic cell cell.3. choice: Selection is finished in hypoxanthine aminopterine thymidine(HAT) medium. Here, in the main 3 kinds of cell are found.*unfused B-cell: which can die within the medium in brief time because it has short life.*unfused malignant tumor cell: which can die within the medium thanks to synthesis stoppage because it is HGPRT- and Ig-.
*hybridoma cell: it’ll survive the medium thanks to B-cell activity.So, somatic cell cell is chosen by this fashion.4.
Screening :It is finished by assay methodology. the chosen cells are transferred to ninety six plastic well plates. every well contains one cell. Specific antigens are adsorbate at the underside of the plates. If the cells turn out desired protein, it’ll bind to it antigens. This protein is then detected by immunoconjugate that contains 2 element.
One element of the immunoconjugate is particular for epitope and protein is immobilized thanks to this element. Another element is catalyst that brings color to the well. once incubation catalyst activity is stopped and optical density is measured by assay reader.
5. biological research : once screening, biological research of the protein will be tired interleukin-6 media for additional growth and production of the antibodies.6.
Characterization and storage: once characterization the antibodies will be keep in N media.