Thymic human corneal epithelial cells (THCEs) and telomerase-immortalized

Thymic stromal lymphopoietin (TSLP) represents a critical factor of adaptive immuneresponses while Toll-like receptors (TLRs) are key players of innate immunitytriggered by Aspergillus fumigatus (AF) in cornea. Here we stimulatedtelomerase-immortalized human corneal epithelial cells (THCEs) andtelomerase-immortalized human stroma fibroblasts (THSFs) with TLR2 Ligandzymosan, TLR4 ligand lipopolysaccharide (LPS), human recombinant TSLP or AFhyphae for various periods, with or without the inhibition of TLR2, TLR4 or TSLPpreviously. It was demonstrated that enhanced expression of TSLP was associatedwith the treatment of A. fumigatus hyphae in human corneal cell lines. Stimulation ofTHCEs and THSFs with zymosan or LPS induced upregulated expression of TSLP;Inhibition of either TLR2 or TLR4 impaired the AF-induced TSLP. The Humanrecombinant TSLP augmented expression of TLR2 and TLR4; Knockdown of TSLPhindered the expression of TLRs, IL-8 and TNF-?.

These findings suggested thatTSLP participated in the adaptive immune response of THCEs and THSFs triggeredby A. fumigatus, which is closely related to the function of TLRs; and the innateimmunity mediated by TLRs could be enhanced by TSLP. Thus, TSLP may serve as alink between the innate and adaptive immune responses in corneal cell lines infectedby Aspergillus fumigatus.

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